Heparin Inhibits the Expression of Interleukin-l1 and Granulocyte- Macrophage Colony-Stimulating Factor in Primate Bone Marrow Stromal Fibroblasts Through mRNA Destabilization

Interactions between different cytokines, extracellular matrix components, and various cell types inside the bone marrow microenvironment are believed to play important roles in the regulation of hematopoiesis. We observed that both interleukin-l (IL-l) and 12-0-tetradecanoylphorbol-13-acetate (TPA) can stimulate the expression of IL-l1 and granulocyte-macrophage colony-stimulating factor (GM-CSF) genes in a primate bone marrow stromal fibroblast cell line, PU34. We also found that IL-lor TPA-stimulated IL-l1 and GM-CSF expression in PU-34 cells can be abolished by heparin, a class of molecules related to extracellular matrix components, glycosaminoglycans. Because the growth inhibitory signals provided by extracellular factors were less understood, the mechanisms of heparin inhibition of IL-l1